coli possesses no regulators controlling PHA biosynthesis.
coli is able to produce PHAs under the nutrient rich condition probably because E. In addition, unlike many natural PHA produces, in which PHA accumulation is induced under the nitrogen and/or phosphate limited conditions, recombinant E. In this regard, a recombinant Escherichia coli system, which incorporates natural and engineered PHA biosynthetic pathways, is useful for achieving high-yield production of various tailor-made PHAs. The primary aims of the metabolic engineering of PHA, therefore, include controlling the different factors that determine these polymer properties and optimizing yield. In addition, the efficient conversion of inexpensive and renewable feedstock into PHA results in value-added products that are competitive with their petroleum counterparts. The material properties of PHAs are governed by their monomer composition, molecular weight and copolymer microstructure.
Polyhydroxyalkanoates (PHAs) are bacterial polyesters that can be developed as commodity plastic materials and also applicable for environmental and biochemical applications. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedĭata Availability: All relevant data are within the paper and its Supporting Information files.įunding: This research was supported by a Core Research for Evolutional Science and Technology (CREST) program “Creation of essential technologies to utilize carbon dioxide as a resource through the enhancement of plant productivity and the exploitation of plant produces” ( ) from the Japan Science and Technology Agency (JST) ( ) (RK and ST). Received: DecemAccepted: MaPublished: June 3, 2015Ĭopyright: © 2015 Kadoya et al. The mtgA deletion should be combined with conventional engineering approaches, and thus, is a promising strategy for improved production of intracellularly accumulated biopolymers.Ĭitation: Kadoya R, Matsumoto K, Ooi T, Taguchi S (2015) MtgA Deletion-Triggered Cell Enlargement of Escherichia coli for Enhanced Intracellular Polyester Accumulation. This result suggests that the increase in volumetric capacity for accumulating intracellular material contributed to the enhanced polymer production. Interestingly, an enlargement in cell width associated with polymer accumulation was observed in this strain, resulting in a 1.6-fold greater polymer accumulation per cell compared to the control. coli JW3175, which was a derivate of superior PHA-producing strain BW25113, was examined for polymer production, and exhibited an enhanced accumulation of the polymer (7.0 g/l) compared to the control (5.2 g/l). The transposon was inserted into the locus within the gene encoding MtgA that takes part, as a non-lethal component, in the formation of the peptidoglycan backbone. A high-throughput screening of polymer-accumulating cells on Nile red-containing plates isolated one mutant that produced 1.8-fold higher quantity of polymer without severe disadvantages in the cell growth and monomer composition of the polymer. coli, which was transformed to produce poly(lactate- co-3-hydroxybutyrate) from glucose. To meet this goal, a transposon-based mutagenesis was carried out on E. The present study aimed at drawing out the latent potential of this organism using genome-wide mutagenesis. Bacterial polyester polyhydroxyalkanoates (PHAs) have been produced in engineered Escherichia coli, which turned into an efficient and versatile platform by applying metabolic and enzyme engineering approaches.
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